NAD+
Essential coenzyme for cellular energy metabolism and DNA repair.
50 mg/day SubQ (conservative maintenance dose)
Key Benefits
serves as the essential substrate for SIRT1–7 enzymes that regulate metabolism, stress resistance, and longevity gene expression
fuels PARP enzymes that detect and repair DNA strand breaks; depletion during damage can be restored with supplementation
maintains the NAD+/NADH ratio critical for the electron transport chain, directly supporting cellular energy output
NAD+ levels oscillate with the circadian clock and regulate SIRT1-mediated transcription of core clock genes
Recommended Injection Sites
Optimal injection locations for NAD+ based on its route (IV, subcutaneous, or oral) and pharmacokinetic profile.
Tap a glowing zone on the body map to highlight the matching site card — or tap a card to activate the zone.
Tap a zone to inject
Produces 8× whole-blood NAD+ increase within 2 hours. Dilute in 250mL NS, infuse over 1–2 hours slowly to minimize flushing. Requires clinical setting.
Achieves 4–5× whole-blood NAD+ increase. Use concentrated solution (100 mg/mL). Practical for maintenance protocols outside clinical settings.
Alternative SubQ site for daily maintenance dosing. Rotate with abdomen.
For SubQ protocols, rotate abdominal quadrants daily. NAD+ infusions must be slow (1–2 hours) — rapid infusion causes uncomfortable flushing and GI symptoms.
- Rapid IV push (causes flushing, nausea, chest tightness)
- Intramuscular injection
- Light exposure of reconstituted solution
Active Signaling Pathway
NAD+ serves as the essential substrate for sirtuin longevity enzymes and PARP DNA repair enzymes. As the rate-limiting molecule in these pathways, replenishment has cascading effects across every cell type in the body.
Recommended Stacks
NAD+ is a key compound in these curated research stacks. Pair it with synergistic peptides for enhanced outcomes.
GH Axis Optimization
Maximize growth hormone output with a dual GHRH approach for serious body composition research.
Longevity & Anti-Aging Performance
Slow the biological clock while maintaining peak physical performance — the ultimate healthspan stack.
Cognitive Performance & Brain Health
Fuel the brain with the compounds that drive neuroplasticity, neuroprotection, and mental clarity.
Total Beauty & Wellness
Inside-out beauty — combining skin repair with the systemic anti-aging compounds that make skin glow from within.
Detox & Glow Protocol
Glutathione 600mg + Lipo-C + NAD+ — deep cellular detox, liver support, and radiant skin from the inside out.
How long will one vial last?
Based on standard research protocols, here's exactly what to expect from a single 500mg vial — and when to plan your next order.
Actual duration may vary based on your specific protocol. Always consult your research guidelines for precise dosing schedules.
Scientific Background
Mechanism of Action
NAD+ is a dinucleotide coenzyme that shuttles electrons in redox reactions (NAD+/NADH couple) and serves as a substrate for three classes of enzymes: sirtuins (SIRT1–7, deacetylases that regulate metabolism and longevity), PARPs (poly-ADP-ribose polymerases, DNA damage sensors), and CD38/CD157 (cyclic ADP-ribose synthases involved in calcium signaling). NAD+ depletion impairs all three pathways simultaneously.
Clinical Context
NAD+ research has exploded in the past decade, driven by the discovery of its central role in sirtuin-mediated longevity pathways. IV and subcutaneous administration bypass the gut absorption limitations of oral precursors (NMN, NR), delivering NAD+ directly to circulation. The 500mg vial supports both single high-dose infusion protocols and multi-dose subcutaneous research schedules.
Research Highlights
- NAD+ levels decline ~50% between ages 40 and 60 in human tissue — one of the most consistent biomarkers of biological aging
- David Sinclair lab (Harvard): NAD+ repletion via NMN restored vascular function and exercise capacity in aged mice to levels of young animals
- PARP activation during DNA damage can deplete cellular NAD+ by up to 80% — supplementation supports repair capacity
- 2023 human trial: IV NAD+ infusion (500mg) increased whole-blood NAD+ by 8× within 2 hours; subcutaneous injection achieves 4–5× increase
Storage & Reconstitution
IV infusion is used in clinical research for rapid repletion. Subcutaneous injection offers a practical alternative with good bioavailability.